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Addgene

psgRNA-GST-EGFP-GPIBY55
(Plasmid #213720)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 213720 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pX330-U6-Chimeric_BB-CBh-hSpCas9
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFP
  • gRNA/shRNA sequence
    Unspecified
  • Species
    H. sapiens (human)
  • Promoter CMV
  • Tag / Fusion Protein
    • GST (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Contain sequence amplified from the pX330-U6-Chimeric_BB-CBh-hSpCas9 (Addgene plasmid #42230).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    psgRNA-GST-EGFP-GPIBY55 was a gift from Qilai Huang (Addgene plasmid # 213720 ; http://n2t.net/addgene:213720 ; RRID:Addgene_213720)

  • For your References section:

    GPI-anchored glutathione S-transferase as marker allows affinity sorting of transfection-positive cells. Ma S, Yang L, Zuo Q, Huang Q. Front Mol Biosci. 2022 Sep 29;9:1016090. doi: 10.3389/fmolb.2022.1016090. eCollection 2022. 10.3389/fmolb.2022.1016090 PubMed 36250010
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