pcDNA3.1-TST-EGFP-GPIBY55
(Plasmid
#213711)
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PurposeFor overexpression of a gene, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIBY55 driven by an SV40 promoter.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 213711 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEGFP
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SpeciesH. sapiens (human)
- Promoter SV40
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Tags
/ Fusion Proteins
- V5-tag (N terminal on insert)
- Twin-strep-tag (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1-TST-EGFP-GPIBY55 was a gift from Qilai Huang (Addgene plasmid # 213711 ; http://n2t.net/addgene:213711 ; RRID:Addgene_213711) -
For your References section:
A Gene Transfer-Positive Cell Sorting System Utilizing Membrane-Anchoring Affinity Tag. Yang L, Cui L, Ma S, Zuo Q, Huang Q. Front Bioeng Biotechnol. 2022 Jun 16;10:930966. doi: 10.3389/fbioe.2022.930966. eCollection 2022. 10.3389/fbioe.2022.930966 PubMed 35782508