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Addgene

R4227X-F
(Plasmid #21371)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21371 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCI (modified)
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4713
  • Vector type
    Mammalian Expression
  • Selectable markers
    none

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5a in dYT at 37oC
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    autosomal dominant polycystic kidney disease type I
  • Alt name
    PKD1 mutant
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    13000
  • Mutation
    Changed R4227 to stop codon
  • GenBank ID
    L33243
  • Entrez Gene
    PKD1 (a.k.a. PBP, PC1, Pc-1, TRPP1)
  • Tag / Fusion Protein
    • FLAG (following D4226) (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SpeI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    R4227X-F was a gift from Gregory Germino (Addgene plasmid # 21371 ; http://n2t.net/addgene:21371 ; RRID:Addgene_21371)

  • For your References section:

    Co-assembly of polycystin-1 and -2 produces unique cation-permeable currents. Hanaoka K, Qian F, Boletta A, Bhunia AK, Piontek K, Tsiokas L, Sukhatme VP, Guggino WB, Germino GG. Nature. . 408(6815):990-4. 10.1038/35050128 PubMed 11140688
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