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Addgene

pCRISPR/eCas9-GPIBY55
(Plasmid #213709)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 213709 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pX330-U6-Chimeric_BB-CBh-hSpCas9
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFP
  • gRNA/shRNA sequence
    Unspecified
  • Species
    H. sapiens (human)
  • Promoter CMV
  • Tags / Fusion Proteins
    • 3XFLAG (N terminal on insert)
    • Twin-strep-tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (destroyed during cloning)
  • 3′ cloning site NotI (destroyed during cloning)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Contain sequence amplified from the pX330-U6-Chimeric_BB-CBh-hSpCas9 (Addgene plasmid #42230).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCRISPR/eCas9-GPIBY55 was a gift from Qilai Huang (Addgene plasmid # 213709 ; http://n2t.net/addgene:213709 ; RRID:Addgene_213709)

  • For your References section:

    A Gene Transfer-Positive Cell Sorting System Utilizing Membrane-Anchoring Affinity Tag. Yang L, Cui L, Ma S, Zuo Q, Huang Q. Front Bioeng Biotechnol. 2022 Jun 16;10:930966. doi: 10.3389/fbioe.2022.930966. eCollection 2022. 10.3389/fbioe.2022.930966 PubMed 35782508
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