PKD1-F (pCI-PKD1-Flag)
(Plasmid #21369)

• Depositing Lab: Gregory Germino
• Publication: Hanaoka et al Nature. . 408(6815):990-4.

Backbone

• Vector backbone: pCI (modified)
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4713
• Vector type: Mammalian Expression
• Selectable markers: none

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5a in dYT at 37oC
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: autosomal dominant polycystic kidney disease type I
• Alt name: PKD1 wild-type
• Species: H. sapiens (human)
• Insert Size (bp): 13117
• Mutation: wild-type sequence*
• GenBank ID: L33243
• Entrez Gene: PKD1 (a.k.a. PBP, PC1, Pc-1, TRPP1)
• Tag / Fusion Protein:
  • FLAG (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SalI (destroyed during cloning)
• 5′ sequencing primer: T7
• 3′ sequencing primer: n/a

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

*Note that the PKD in this plasmid does have a T3206C, which changes a serine to a proline. From the depositor: "As far as [we] know, this missense change is not pathologic and the cDNA including this change appears to be functional using several assays but it is not the standard PKD1 sequence in the database."