WT (REP10)
(Plasmid #21368)

• Depositing Lab: Gregory Germino
• Publication: Qian et al Proc Natl Acad Sci U S A. 2002 Dec 24. 99(26):16981-6.

Backbone

• Vector backbone: pREP10
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 8500
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5a in dYT at 37oC
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: autosomal dominant polycystic kidney disease type I
• Alt name: PKD1 wild-type
• Species: H. sapiens (human)
• Insert Size (bp): 14000
• GenBank ID: L33243
• Entrez Gene: PKD1 (a.k.a. PBP, PC1, Pc-1, TRPP1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (destroyed during cloning)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry