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Addgene

pET SUMO-syt1 5W
(Plasmid #213623)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 213623 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET SUMO
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Use BL21 cells for protein expression.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    syt1 5W
  • Species
    R. norvegicus (rat)
  • Mutation
    M173W, F231W, F234W, C277A, V304W, Y364W, I367C
  • Entrez Gene
    Syt1 (a.k.a. P65)
  • Tag / Fusion Protein
    • SUMO (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET SUMO-syt1 5W was a gift from Edwin Chapman (Addgene plasmid # 213623 ; http://n2t.net/addgene:213623 ; RRID:Addgene_213623)

  • For your References section:

    Synaptotagmin-7 outperforms synaptotagmin-1 to promote the formation of large, stable fusion pores via robust membrane penetration. Courtney KC, Mandal T, Mehta N, Wu L, Li Y, Das D, Cui Q, Chapman ER. Nat Commun. 2023 Nov 27;14(1):7761. doi: 10.1038/s41467-023-42497-8. 10.1038/s41467-023-42497-8 PubMed 38012142
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