SPLICS LY-MT Short P2A
(Plasmid
#213614)
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PurposeDetect the short-range Lysosome-Mitochondria contact
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 213614 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
- Backbone size w/o insert (bp) 5387
- Total vector size (bp) 7130
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSPLICS LY-MT Short P2A
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SpeciesH. sapiens (human)
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Insert Size (bp)1743
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SPLICS LY-MT Short P2A was a gift from Tito Calì (Addgene plasmid # 213614 ; http://n2t.net/addgene:213614 ; RRID:Addgene_213614) -
For your References section:
A SPLICS reporter reveals [Formula: see text]-synuclein regulation of lysosome-mitochondria contacts which affects TFEB nuclear translocation. Giamogante F, Barazzuol L, Maiorca F, Poggio E, Esposito A, Masato A, Napolitano G, Vagnoni A, Cali T, Brini M. Nat Commun. 2024 Feb 19;15(1):1516. doi: 10.1038/s41467-024-46007-2. 10.1038/s41467-024-46007-2 PubMed 38374070