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Addgene

pT2/Onc
(Plasmid #21360)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21360 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    unknown
  • Backbone size w/o insert (bp) 2918
  • Vector type
    transposon

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sleeping Beauty Transposon with splice acceptors and murine stem cell virus LTR
  • Alt name
    T2-Onc
  • Alt name
    Oncogenic Sleeping Beauty Transposon
  • Insert Size (bp)
    2163

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT2/Onc was a gift from David Largaespada (Addgene plasmid # 21360 ; http://n2t.net/addgene:21360 ; RRID:Addgene_21360)

  • For your References section:

    Cancer gene discovery in solid tumours using transposon-based somatic mutagenesis in the mouse. Collier LS, Carlson CM, Ravimohan S, Dupuy AJ, Largaespada DA. Nature. 2005 Jul 14. 436(7048):272-6. 10.1038/nature03681 PubMed 16015333
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