WT (AF20)
(Plasmid #21359)

• Depositing Lab: Gregory Germino
• Publication: Qian et al Proc Natl Acad Sci U S A. 2002 Dec 24. 99(26):16981-6.

Backbone

• Vector backbone: pCI (modified)
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4713
• Vector type: Mammalian Expression
• Selectable markers: none

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5a in dYT at 37oC
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: autosomal dominant polycystic kidney disease type I
• Alt name: PKD1 wild-type
• Species: H. sapiens (human)
• Insert Size (bp): 13117
• Mutation: wild-type sequence*
• GenBank ID: L33243
• Entrez Gene: PKD1 (a.k.a. PBP, PC1, Pc-1, TRPP1)
• Tag / Fusion Protein:
  • FLAG (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SalI (destroyed during cloning)
• 5′ sequencing primer: T7 EEV
• 3′ sequencing primer: n/a

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

*Please note: during Addgene's quality control process, the following mutations were found in PKD1: A691P, M792L, S999P, N1056T, T1724A, M1976V. The depositor has noted that the plasmid appears to be functional using several assays but it is not the standard PKD1 sequence in the database (Genbank ID L33243).