pInducer-TAZ-S89A/S311A
(Plasmid
#213589)
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PurposeDoxycycline inducible expression of TAZ-S89A/S311A cDNA in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 213589 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonep-Inducer10b
- Backbone size w/o insert (bp) 12153
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTAZ
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SpeciesH. sapiens (human)
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Insert Size (bp)1272
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MutationS89A, S311A
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Entrez GeneWWTR1 (a.k.a. TAZ)
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Tag
/ Fusion Protein
- 2xHA (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Age1 (not destroyed)
- 3′ cloning site Mlu1 (not destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pInducer-TAZ-S89A/S311A was a gift from Channing Der (Addgene plasmid # 213589 ; http://n2t.net/addgene:213589 ; RRID:Addgene_213589) -
For your References section:
TEAD Inhibition Overcomes YAP1/TAZ-driven Primary and Acquired Resistance to KRASG12C Inhibitors. Edwards AC, Stalnecker CA, Jean Morales A, Taylor KE, Klomp JE, Klomp JA, Waters AM, Sudhakar N, Hallin J, Tang TT, Olson P, Post L, Christensen JG, Cox AD, Der CJ. Cancer Res. 2023 Nov 7. doi: 10.1158/0008-5472.CAN-23-2994. 10.1158/0008-5472.CAN-23-2994 PubMed 37934103
