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Addgene

pLv-EF1a-Parkin(W403A)-A92mKO2
(Plasmid #213558)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 213558 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Addgene #85132
  • Backbone size w/o insert (bp) 7438
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Parkin
  • Alt name
    PARK2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2091
  • Mutation
    W403A mutation in Parkin
  • Entrez Gene
    PRKN (a.k.a. AR-JP, LPRS2, PARK2, PDJ)
  • Promoter EF1a

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MluI (not destroyed)
  • 3′ cloning site NsiI (not destroyed)
  • 5′ sequencing primer EF-1a Forward
  • 3′ sequencing primer WPRE-R
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    mKO2 from Addgene #83841. Parkin from Addgene #23956.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Parkin Internally tagged with mKO2 following Alanine 92.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLv-EF1a-Parkin(W403A)-A92mKO2 was a gift from Steven Altschuler & Lani Wu (Addgene plasmid # 213558 ; http://n2t.net/addgene:213558 ; RRID:Addgene_213558)

  • For your References section:

    A PINK1 input threshold arises from positive feedback in the PINK1/Parkin mitophagy decision circuit. Waters CS, Angenent SB, Altschuler SJ, Wu LF. Cell Rep. 2023 Oct 31;42(10):113260. doi: 10.1016/j.celrep.2023.113260. Epub 2023 Oct 17. 10.1016/j.celrep.2023.113260 PubMed 37851575
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