pLv-EF1a-Parkin(K161N)-A92mKO2
(Plasmid
#213554)
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PurposeLentiviral vector for stable expression of K161N Parkin, internally tagged with mKO2
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 213554 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneAddgene #85132
- Backbone size w/o insert (bp) 7438
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameParkin
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Alt namePARK2
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SpeciesH. sapiens (human)
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Insert Size (bp)2091
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MutationK161N mutation in Parkin
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Entrez GenePRKN (a.k.a. AR-JP, LPRS2, PARK2, PDJ)
- Promoter EF1a
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site MluI (not destroyed)
- 3′ cloning site NsiI (not destroyed)
- 5′ sequencing primer EF-1a Forward
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bymKO2 from Addgene #83841. Parkin from Addgene #23956.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Parkin Internally tagged with mKO2 following Alanine 92.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLv-EF1a-Parkin(K161N)-A92mKO2 was a gift from Steven Altschuler & Lani Wu (Addgene plasmid # 213554 ; http://n2t.net/addgene:213554 ; RRID:Addgene_213554) -
For your References section:
A PINK1 input threshold arises from positive feedback in the PINK1/Parkin mitophagy decision circuit. Waters CS, Angenent SB, Altschuler SJ, Wu LF. Cell Rep. 2023 Oct 31;42(10):113260. doi: 10.1016/j.celrep.2023.113260. Epub 2023 Oct 17. 10.1016/j.celrep.2023.113260 PubMed 37851575