pCMV-Parkin(E16A)
(Plasmid
#213546)
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PurposeTransient mammalian expresssion of E16A Parkin
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 213546 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneAddgene #23956
- Backbone size w/o insert (bp) 3954
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameParkin
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Alt namePARK2
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SpeciesH. sapiens (human)
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Insert Size (bp)1398
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MutationE16A mutation in Parkin
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Entrez GenePRKN (a.k.a. AR-JP, LPRS2, PARK2, PDJ)
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV Forward (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-Parkin(E16A) was a gift from Steven Altschuler & Lani Wu (Addgene plasmid # 213546 ; http://n2t.net/addgene:213546 ; RRID:Addgene_213546) -
For your References section:
A PINK1 input threshold arises from positive feedback in the PINK1/Parkin mitophagy decision circuit. Waters CS, Angenent SB, Altschuler SJ, Wu LF. Cell Rep. 2023 Oct 31;42(10):113260. doi: 10.1016/j.celrep.2023.113260. Epub 2023 Oct 17. 10.1016/j.celrep.2023.113260 PubMed 37851575