pCMV-mKO2-Parkin
(Plasmid
#213544)
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PurposeTransient mammalian expresssion of Parkin, N-terminally tagged with mKO2, M1 of Parkin intact
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 213544 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneAddgene #23956
- Backbone size w/o insert (bp) 3954
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameParkin
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Alt namePARK2
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SpeciesH. sapiens (human)
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Insert Size (bp)2100
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Entrez GenePRKN (a.k.a. AR-JP, LPRS2, PARK2, PDJ)
- Promoter CMV
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Tag
/ Fusion Protein
- mKO2 (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV Forward (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bymKO2 from Addgen #83841
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Backbone source used an M1L substitution in Parkin, corrected here.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-mKO2-Parkin was a gift from Steven Altschuler & Lani Wu (Addgene plasmid # 213544 ; http://n2t.net/addgene:213544 ; RRID:Addgene_213544) -
For your References section:
A PINK1 input threshold arises from positive feedback in the PINK1/Parkin mitophagy decision circuit. Waters CS, Angenent SB, Altschuler SJ, Wu LF. Cell Rep. 2023 Oct 31;42(10):113260. doi: 10.1016/j.celrep.2023.113260. Epub 2023 Oct 17. 10.1016/j.celrep.2023.113260 PubMed 37851575