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pOTTC1992 - pAAV EF1a CoV2 Orf3a-2xStrep
(Plasmid #213542)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 213542 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pOTTC1464 - pAAV EF1a NheI iRFP-FLAG AscI
  • Backbone manufacturer
    NIDA GEVVC
  • Backbone size w/o insert (bp) 5396
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CoV2 Orf3a-2xStrep
  • Species
    H. sapiens (human); Severe acute respiratory syndrome coronavirus 2
  • Insert Size (bp)
    930
  • Entrez Gene
    ORF3a (a.k.a. GU280_gp03)
  • Promoter EF1a

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer EF-1a-Forward TCAAGCCTCAGACAGTGGTTC
  • 3′ sequencing primer WPRE R1 CATAGCGTAAAAGGAGCAACA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOTTC1992 - pAAV EF1a CoV2 Orf3a-2xStrep was a gift from Brandon Harvey & Christopher Richie (Addgene plasmid # 213542 ; http://n2t.net/addgene:213542 ; RRID:Addgene_213542)
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