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pRSF_his6_sumo_hRNMT_RAM
(Plasmid #213528)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 213528 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSF-His6-sumo
  • Backbone size w/o insert (bp) 3900
  • Total vector size (bp) 5900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    human RNMT
  • Alt name
    RNMT
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1431
  • Entrez Gene
    RNMT (a.k.a. CMT1, CMT1c, MET, Met, N7-MTase, RG7MT1, cm1p, hCMT1, hMet)
  • Tag / Fusion Protein
    • His6-sumo (N terminal on backbone)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site AscI (not destroyed)
  • 5′ sequencing primer GGCTGATGGAAGCGTTCGCTA
  • 3′ sequencing primer T7-term
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    RAM
  • Alt name
    RAM
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    357

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer GGCTGATGGAAGCGTTCGCTA
  • 3′ sequencing primer T7-term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSF_his6_sumo_hRNMT_RAM was a gift from Thomas Tuschl (Addgene plasmid # 213528 ; http://n2t.net/addgene:213528 ; RRID:Addgene_213528)

  • For your References section:

    Small-molecule inhibition of SARS-CoV-2 NSP14 RNA cap methyltransferase. Meyer C, Garzia A, Miller MW, Huggins DJ, Myers RW, Hoffmann HH, Ashbrook AW, Jannath SY, Liverton N, Kargman S, Zimmerman M, Nelson AM, Sharma V, Dolgov E, Cangialosi J, Penalva-Lopez S, Alvarez N, Chang CW, Oswal N, Gonzalez I, Rasheed R, Goldgirsh K, Davis JA, Ramos-Espiritu L, Menezes MR, Larson C, Nitsche J, Ganichkin O, Alwaseem H, Molina H, Steinbacher S, Glickman JF, Perlin DS, Rice CM, Meinke PT, Tuschl T. Nature. 2024 Dec 11. doi: 10.1038/s41586-024-08320-0. 10.1038/s41586-024-08320-0 PubMed 39663451
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