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Addgene

eGFP-SUN1
(Plasmid #213515)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 213515 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Thermo Fisher
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 8842
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SUN1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3399
  • Mutation
    SUN1 has a silent mutation on its gene (nucleotide 1275 (C->T)). Confers resistance to siSUN1 with the sequence CCGTGTTGAATTGGGCAAGCA.
  • GenBank ID
    AB648918.1
  • Entrez Gene
    SUN1 (a.k.a. UNC84A)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CGC AAA TGG GCG GTA GGC GTG
  • 3′ sequencing primer TAG AAG GCA CAG TCG AGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    eGFP-SUN1 was a gift from Karim Mekhail (Addgene plasmid # 213515 ; http://n2t.net/addgene:213515 ; RRID:Addgene_213515)

  • For your References section:

    DNA double-strand break-capturing nuclear envelope tubules drive DNA repair. Shokrollahi M, Stanic M, Hundal A, Chan JNY, Urman D, Hakem A, Garcia RE, Hao J, Maass PG, Dickson BC, Hande MP, Pujana MA, Hakem R, Mekhail K. bioRxiv 2023.05.07.539750 10.1101/2023.05.07.539750
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