pCK123 pDESTattB_exorh:mCerulean
(Plasmid
#213512)
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Purpose(Empty Backbone) attB-containing pDEST plasmid, for use in Multisite Gateway reaction
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 213512 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDESTattB
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Backbone manufacturerInvitrogen
- Backbone size (bp) 7064
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Vector typeZebrafish Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberLow Copy
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2023.12.08.570868 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCK123 pDESTattB_exorh:mCerulean was a gift from Christian Mosimann (Addgene plasmid # 213512 ; http://n2t.net/addgene:213512 ; RRID:Addgene_213512) -
For your References section:
pIGLET: Safe harbor landing sites for reproducible and efficient transgenesis in zebrafish. Lalonde RL, Wells HH, Kemmler CL, Nieuwenhuize S, Lerma R, Burger A, Mosimann C. Sci Adv. 2024 Jun 7;10(23):eadn6603. doi: 10.1126/sciadv.adn6603. Epub 2024 Jun 5. 10.1126/sciadv.adn6603 PubMed 38838146
Map uploaded by the depositor.
