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pJG01
(Plasmid #213505)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 213505 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJP72
  • Backbone manufacturer
    Duojia Pan (based on pUC57-mini from Genscript)
  • Backbone size w/o insert (bp) 4221
  • Total vector size (bp) 5652
  • Modifications to backbone
    TdTomato was codon-optimized for Capsaspora owczarzaki and synthesized. The mScarlet gene ORF of pJP72 was directly replaced with the ORF of this TdTomato gene
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TdTomato
  • Species
    Synthetic; ; Capsaspora owczarzaki
  • Insert Size (bp)
    1431
  • Promoter EF1

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer GATTTGGAAATGCTCACTCTTCC
  • 3′ sequencing primer TGAAATAAACAAATGTGTCTTGGC
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    pJP72 was a gift from Duojia Pan (Addgene plasmid # 176479 ; http://n2t.net/addgene:176479 ; RRID:Addgene_176479)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJG01 was a gift from Joseph Gerdt (Addgene plasmid # 213505 ; http://n2t.net/addgene:213505 ; RRID:Addgene_213505)
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