pET-C
(Plasmid
#213496)
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PurposeEncodes Node C of the three-node Turing circuit
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 213496 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBR322
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert 1
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Gene/Insert namelambda cI 5G6GP
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Alt namelexa family transcriptional regulator
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Insert Size (bp)756
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Tag
/ Fusion Protein
- LVA tag (C terminal on insert)
Gene/Insert 2
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Gene/Insert namemCherry
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Insert Size (bp)753
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Tag
/ Fusion Protein
- ASV tag (C terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET-C was a gift from Mark Isalan (Addgene plasmid # 213496 ; http://n2t.net/addgene:213496 ; RRID:Addgene_213496)