pCDF-B-pC
(Plasmid
#213495)
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PurposeEncodes Node B of the three-node Turing circuit
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 213495 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCloDF13
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert 1
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Gene/Insert namecinI
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Insert Size (bp)705
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Entrez GenecinI (a.k.a. RHECIAT_CH0003073)
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Tag
/ Fusion Protein
- LVA tag (C terminal on insert)
Gene/Insert 2
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Gene/Insert namelacI
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Insert Size (bp)1125
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Entrez GenelacI (a.k.a. b0345, ECK0342)
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Tag
/ Fusion Protein
- LVA tag (C terminal on insert)
Gene/Insert 3
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Gene/Insert namelambda cI
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Alt namelexa family transcriptional regulator
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Insert Size (bp)756
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Entrez Genelambdap50 (a.k.a. lambdap50)
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Tag
/ Fusion Protein
- LVA tag (C terminal on insert)
Gene/Insert 4
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Gene/Insert namesfGFP
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Alt namesuperfolder GFP
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Insert Size (bp)759
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Tag
/ Fusion Protein
- ASV tag (C terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDF-B-pC was a gift from Mark Isalan (Addgene plasmid # 213495 ; http://n2t.net/addgene:213495 ; RRID:Addgene_213495)