pCOLA-A-pC-tac
(Plasmid
#213494)
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PurposeEncodes Node A of the three-node Turing circuit
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 213494 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCOLA
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert 1
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Gene/Insert namerpaI
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Insert Size (bp)699
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Entrez GenerpaI (a.k.a. I8G32_RS01660, I8G32_00333)
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Tag
/ Fusion Protein
- ASV tag (C terminal on insert)
Gene/Insert 2
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Gene/Insert nametetR
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Insert Size (bp)663
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Tag
/ Fusion Protein
- LVA tag (C terminal on insert)
Gene/Insert 3
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Gene/Insert name4cl
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Alt nameferuloyl-CoA synthase
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SpeciesRhodopseudomonas palustris
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Insert Size (bp)1896
Gene/Insert 4
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Gene/Insert nametal
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Alt namearomatic amino acid ammonia-lyase
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SpeciesCereibacter sphaeroides
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Insert Size (bp)1572
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCOLA-A-pC-tac was a gift from Mark Isalan (Addgene plasmid # 213494 ; http://n2t.net/addgene:213494 ; RRID:Addgene_213494)