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Addgene

pYPQ265E6
(Plasmid #213469)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 213469 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYPQ166
  • Vector type
    Plant Expression, CRISPR ; Gateway compatible TadA-CDa-32aa linker-zSpCas9-D10A-2xUGI entry clone

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TadA-CDa-32aa linker-zSpCas9-D10A-2xUGI
  • Tags / Fusion Proteins
    • No
    • No

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.02.25.581917 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYPQ265E6 was a gift from Yiping Qi (Addgene plasmid # 213469 ; http://n2t.net/addgene:213469 ; RRID:Addgene_213469)

  • For your References section:

    High performance TadA-8e derived cytosine and dual base editors with undetectable off-target effects in plants. Fan T, Cheng Y, Wu Y, Liu S, Tang X, He Y, Liao S, Zheng X, Zhang T, Qi Y, Zhang Y. Nat Commun. 2024 Jun 14;15(1):5103. doi: 10.1038/s41467-024-49473-w. 10.1038/s41467-024-49473-w PubMed 38877035
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