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Addgene

Fg vector
(Plasmid #213464)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 213464 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Fg vector
  • Backbone manufacturer
    Hammond-Kosack' lab
  • Backbone size (bp) 4497
  • Modifications to backbone
    The resistance cassette and the origin of replication were amplified from pGreen. Then, the amplicon was ligated to a fragment containing: the right border of the TSI locus 1, a cloning site for Golden Gate cloning approach and the partial sequence of the geneticin selection marker.
  • Vector type
    Bacterial Expression
  • Selectable markers
    Geneticin

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Fg vector was a gift from Kim Hammond-Kosack (Addgene plasmid # 213464 ; http://n2t.net/addgene:213464 ; RRID:Addgene_213464)

  • For your References section:

    Identification and functional characterisation of a locus for target site integration in Fusarium graminearum. Darino M, Urban M, Kaur N, Machado Wood A, Grimwade-Mann M, Smith D, Beacham A, Hammond-Kosack K. Fungal Biol Biotechnol. 2024 Feb 26;11(1):2. doi: 10.1186/s40694-024-00171-8. 10.1186/s40694-024-00171-8 PubMed 38409036
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