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Addgene

Flag-SKP1-RR (K142R/K163R)
(Plasmid #213425)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 213425 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human Flag tagged SKP1-K142R/K163R
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    500
  • Entrez Gene
    SKP1 (a.k.a. EMC19, OCP-II, OCP2, SKP1A, TCEB1L, p19A)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Flag-SKP1-RR (K142R/K163R) was a gift from Michele Pagano (Addgene plasmid # 213425 ; http://n2t.net/addgene:213425 ; RRID:Addgene_213425)

  • For your References section:

    A noncanonical function of SKP1 regulates the switch between autophagy and unconventional secretion. Li J, Krause GJ, Gui Q, Kaushik S, Rona G, Zhang Q, Liang FX, Dhabaria A, Anerillas C, Martindale JL, Vasilyev N, Askenazi M, Ueberheide B, Nudler E, Gorospe M, Cuervo AM, Pagano M. Sci Adv. 2023 Oct 13;9(41):eadh1134. doi: 10.1126/sciadv.adh1134. Epub 2023 Oct 13. 10.1126/sciadv.adh1134 PubMed 37831778
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