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pcDNA3.1-VcnCS-E1015A-E1021A
(Plasmid #213414)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 213414 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 5333
  • Total vector size (bp) 9991
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    VcnCS-E1015A-E1021A
  • Alt name
    VinCS-E1015A-E1021A
  • Alt name
    VinculinCS-E1015A-E1021A
  • Species
    G. gallus (chicken), Synthetic
  • Insert Size (bp)
    4658
  • Mutation
    Mutated vinculin glutamic acid 1015 and 1021 to alanine (E1015A and E1021A)
  • Entrez Gene
    VCL (a.k.a. VINC1)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer BGH Reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-VcnCS-E1015A-E1021A was a gift from Brenton Hoffman (Addgene plasmid # 213414 ; http://n2t.net/addgene:213414 ; RRID:Addgene_213414)

  • For your References section:

    Molecular basis and cellular functions of vinculin-actin directional catch bonding. Chirasani VR, Khan MAI, Malavade JN, Dokholyan NV, Hoffman BD, Campbell SL. Nat Commun. 2023 Dec 14;14(1):8300. doi: 10.1038/s41467-023-43779-x. 10.1038/s41467-023-43779-x PubMed 38097542
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