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pHTN_HaloTag_KIF5B
(Plasmid #213404)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 213404 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHTN HaloTag CMV-neo
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 6143
  • Total vector size (bp) 8959
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    HaloTag-KIF5B
  • Alt name
    Human kinesin-1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3822
  • GenBank ID
    3799
  • Entrez Gene
    KIF5B (a.k.a. HEL-S-61, KINH, KNS, KNS1, UKHC)
  • Promoter CMV
  • Tag / Fusion Protein
    • HaloTag (N terminal on insert)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHTN_HaloTag_KIF5B was a gift from Jonas Ries (Addgene plasmid # 213404 ; http://n2t.net/addgene:213404 ; RRID:Addgene_213404)

  • For your References section:

    Direct observation of motor protein stepping in living cells using MINFLUX. Deguchi T, Iwanski MK, Schentarra EM, Heidebrecht C, Schmidt L, Heck J, Weihs T, Schnorrenberg S, Hoess P, Liu S, Chevyreva V, Noh KM, Kapitein LC, Ries J. Science. 2023 Mar 10;379(6636):1010-1015. doi: 10.1126/science.ade2676. Epub 2023 Mar 9. 10.1126/science.ade2676 PubMed 36893247
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