pAAV-hSyn-DIO-myr-EGFP-LDLR(Ct)
(Plasmid
#213402)
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Purposedouple floxed, myristoylation (myr) site for membrane targeting and EGFP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 213402 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV
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Backbone manufacturerDeisseroth Lab
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEGFP
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Alt namemyr-EGFP-LDLR(Ct)
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Tags
/ Fusion Proteins
- myristoylation site (N terminal on insert)
- C-terminal (Ct) cytoplasmic domains of low density lipoprotein receptor (C terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-hSyn-DIO-myr-EGFP-LDLR(Ct) was a gift from Katrin Willig (Addgene plasmid # 213402 ; http://n2t.net/addgene:213402 ; RRID:Addgene_213402) -
For your References section:
Environmental enrichment enhances patterning and remodeling of synaptic nanoarchitecture as revealed by STED nanoscopy. Wegner W, Steffens H, Gregor C, Wolf F, Willig KI. Elife. 2022 Feb 23;11:e73603. doi: 10.7554/eLife.73603. 10.7554/eLife.73603 PubMed 35195066