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Addgene

pLKO mouse shRNA 1 raptor
(Plasmid #21339)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21339 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLKO.1
  • Backbone manufacturer
    Available from Addgene
  • Backbone size w/o insert (bp) 7032
  • Vector type
    Mammalian Expression, Lentiviral, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL10 Gold
  • Growth instructions
    XL10 Gold Ultracompetent Cells from Stratagene or nStb13. Grow in SOC media at 37oC
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    raptor shRNA 1
  • Alt name
    raptor
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    60
  • GenBank ID
    NM_028898
  • Entrez Gene
    Rptor (a.k.a. 4932417H02Rik, Rap, Raptor, mKIAA1303)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer LKO.1 5'
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

mouse raptor shRNA 1.

shRNA oligo sequence is 5'- CCGGCCTCATCGTCAAGTCCTTCAACTCGAGTTGAAGGACTTGACGATGAGGTTTTTG -3'

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLKO mouse shRNA 1 raptor was a gift from David Sabatini (Addgene plasmid # 21339 ; http://n2t.net/addgene:21339 ; RRID:Addgene_21339)

  • For your References section:

    An ATP-competitive mammalian target of rapamycin inhibitor reveals rapamycin-resistant functions of mTORC1. Thoreen CC, Kang SA, Chang JW, Liu Q, Zhang J, Gao Y, Reichling LJ, Sim T, Sabatini DM, Gray NS. J Biol Chem. 2009 Mar 20. 284(12):8023-32. 10.1074/jbc.M900301200 PubMed 19150980
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