PTAFR-DuET
(Plasmid
#213368)
-
PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 213368 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepcDNA 3.1 (+)
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namePTAFR
-
Alt namePlatelet activating factor receptor
-
SpeciesH. sapiens (human)
-
Entrez GenePTAFR (a.k.a. PAFR)
- Promoter CMV, T7
-
Tags
/ Fusion Proteins
- FLAG (N terminal on insert)
- 1D4 (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Class: A, Family: Platelet activating factor. Ensembl ID: ENSG00000169403, Uniprot ID: P25105
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
PTAFR-DuET was a gift from Thomas Sakmar (Addgene plasmid # 213368 ; http://n2t.net/addgene:213368 ; RRID:Addgene_213368) -
For your References section:
Multiplexed selectivity screening of anti-GPCR antibodies. Dahl L, Kotliar IB, Bendes A, Dodig-Crnkovic T, Fromm S, Elofsson A, Uhlen M, Sakmar TP, Schwenk JM. Sci Adv. 2023 May 3;9(18):eadf9297. doi: 10.1126/sciadv.adf9297. Epub 2023 May 3. 10.1126/sciadv.adf9297 PubMed 37134173