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Addgene

HCA2-DuET
(Plasmid #213308)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 213308 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA 3.1 (+)
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HCA2
  • Alt name
    Hydroxycarboxylic acid receptor 2
  • Alt name
    GPR109A, HCA2, HM74A, NIACR1, Puma-g, PUMAG
  • Species
    H. sapiens (human)
  • Entrez Gene
    HCAR2 (a.k.a. GPR109A, HCA2, HM74a, HM74b, NIACR1, PUMAG, Puma-g)
  • Promoter CMV, T7
  • Tags / Fusion Proteins
    • FLAG (N terminal on insert)
    • 1D4 (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Class: A, Family: Hydroxycarboxylic acid. Ensembl ID: ENSG00000182782, Uniprot ID: Q8TDS4

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HCA2-DuET was a gift from Thomas Sakmar (Addgene plasmid # 213308 ; http://n2t.net/addgene:213308 ; RRID:Addgene_213308)

  • For your References section:

    Multiplexed selectivity screening of anti-GPCR antibodies. Dahl L, Kotliar IB, Bendes A, Dodig-Crnkovic T, Fromm S, Elofsson A, Uhlen M, Sakmar TP, Schwenk JM. Sci Adv. 2023 May 3;9(18):eadf9297. doi: 10.1126/sciadv.adf9297. Epub 2023 May 3. 10.1126/sciadv.adf9297 PubMed 37134173
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