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Addgene

pLNC Int3HA
(Plasmid #21329)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21329 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pLNC
  • Backbone size w/o insert (bp) 6700
  • Vector type
    Retroviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Int3
  • Alt name
    Notch4/Int3
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2400
  • Entrez Gene
    Notch4 (a.k.a. Int-3, Int3, N4)
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLNC Int3HA was a gift from Jan Kitajewski (Addgene plasmid # 21329 ; http://n2t.net/addgene:21329 ; RRID:Addgene_21329)
  • For your References section:

    Notch4 and Wnt-1 proteins function to regulate branching morphogenesis of mammary epithelial cells in an opposing fashion. Uyttendaele H, Soriano JV, Montesano R, Kitajewski J. Dev Biol. 1998 Apr 15. 196(2):204-17. 10.1006/dbio.1998.8863 PubMed 9576833