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pGL3-Control-PTEN-3'UTR-Mut
(Plasmid #21327)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21327 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL3-Control
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5256
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PTEN 3' UTR Mutant
  • Alt name
    PTEN
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    865
  • Mutation
    2 miR binding site mutated. Changed from TTTGCAC to TGTTCTC.
  • Entrez Gene
    PTEN (a.k.a. 10q23del, BZS, CWS1, DEC, GLM2, MHAM, MMAC1, PTEN1, PTENbeta, TEP1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer Luc-F (5'-CAGTCAAGTAACAACCGCGA-3')
  • 3′ sequencing primer EBV-rev
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3-Control-PTEN-3'UTR-Mut was a gift from Joshua Mendell (Addgene plasmid # 21327 ; http://n2t.net/addgene:21327 ; RRID:Addgene_21327)

  • For your References section:

    c-Myc-regulated microRNAs modulate E2F1 expression. O'Donnell KA, Wentzel EA, Zeller KI, Dang CV, Mendell JT. Nature. 2005 Jun 9. 435(7043):839-43. 10.1038/nature03677 PubMed 15944709
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