GHSR-DuET
(Plasmid #213251)

• Purpose: The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
• Depositing Lab: Thomas Sakmar
• Publication: Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi: 10.1126/sciadv.adf9297. Epub 2023 May 3.

Backbone

• Vector backbone: pcDNA 3.1 (+)
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GHSR
• Alt name: Growth hormone secretagogue receptor
• Species: H. sapiens (human)
• Entrez Gene: GHSR (a.k.a. GHDP, GHS-R1a, GHSR-1a)
• Promoter: CMV, T7
• Tags / Fusion Proteins:
  • FLAG (N terminal on insert)
  • 1D4 (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Class: A, Family: Ghrelin. Ensembl ID: ENSG00000121853, Uniprot ID: Q92847