pHR'-ROMK1-CITE-GFP
(Plasmid #21322)

• Depositing Lab: Jay Nadeau
• Publication: Nadeau et al J Neurophysiol. 2000 Aug . 84(2):1062-75.

Backbone

• Vector backbone: pHR'
• Backbone manufacturer: Didier Trono
• Backbone size w/o insert (bp): 10000
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ROMK1
• Species: R. norvegicus (rat)
• Insert Size (bp): 3375
• GenBank ID: NM_017023
• Entrez Gene: Kcnj1 (a.k.a. KAB-1, Kcnj, Kcnj1_v1, Kcnj1_v3, ROMK1, ROMK2, ROMK3, kir1.1)
• Tag / Fusion Protein:
  • GFP (bicistronic with CITE/IRES)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (destroyed during cloning)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Kevin Ho Colin G. Nichols W. Jonathan Lederer Jonathan Lytton Peter M. Vassilev Marie V. Kanazirska & Steven C. Hebert Nature 362, 31 - 38 (1993)
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry