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PL-SIN-EF1α-EGFP
(Plasmid #21320)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21320 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PL-SIN lentiviral vector
  • Backbone manufacturer
    Buzina et al., PLoS Genetics, 2008
  • Backbone size w/o insert (bp) 6881
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Enhanced Green Fluorescence Protein
  • Alt name
    EGFP
  • Insert Size (bp)
    720
  • Promoter EF1α

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

EGFP driven by ubiquitous EF1α promoter. PL-SIN lentiviral vector (Buzina et al., PLoS Genetics, 2008) requires Tat cDNA for lentivirus production, as well as other packaging cDNAs (Gag/pol, Rev, and VSV-G).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PL-SIN-EF1α-EGFP was a gift from James Ellis (Addgene plasmid # 21320 ; http://n2t.net/addgene:21320 ; RRID:Addgene_21320)

  • For your References section:

    Isolation of human iPS cells using EOS lentiviral vectors to select for pluripotency. Hotta A, Cheung AY, Farra N, Vijayaragavan K, Seguin CA, Draper JS, Pasceri P, Maksakova IA, Mager DL, Rossant J, Bhatia M, Ellis J. Nat Methods. 2009 May . 6(5):370-6. 10.1038/nmeth.1325 PubMed 19404254
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