PL-SIN-EOS-S(4+)-EGFP
(Plasmid #21317)

• Purpose: Lentiviral EOS reporter with Sox2 enhancer (x4), expresses EGFP
• Depositing Lab: James Ellis
• Publication: Hotta et al Nat Methods. 2009 May . 6(5):370-6.

Backbone

• Vector backbone: PL-SIN lentiviral vector
• Backbone manufacturer: Buzina et al., PLoS Genetics, 2008
• Backbone size w/o insert (bp): 6531
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Enhanced Green Fluorescence Protein
• Alt name: EGFP
• Insert Size (bp): 720
• Promoter: EOS-S(4+) promoter region

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

EGFP driven by EOS-S(4+) promoter. PL-SIN lentiviral vector (Buzina et al., PLoS Genetics, 2008) requires Tat cDNA for lentivirus production, as well as other packaging cDNAs (Gag/pol, Rev, and VSV-G).