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pCDF-Leup
(Plasmid #213154)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 213154 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDFDuet-1
  • Backbone manufacturer
    Novagen (EMD Millipore)
  • Backbone size w/o insert (bp) 3781
  • Total vector size (bp) 9421
  • Modifications to backbone
    Insert the Leupeptin Protease Inhibitor Pathway
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Streptomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Leupeptin Protease Inhibitor Pathway
  • Alt name
    LeupA, LeupB, LeupC, LeupD
  • Species
    Xenorhabdus bovienii SS-2004
  • Insert Size (bp)
    5733
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site AvrII (not destroyed)
  • 5′ sequencing primer ACYCDuetUP1
  • 3′ sequencing primer DuetDOWN1
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Jhe-Hao Li

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDF-Leup was a gift from Jason Crawford (Addgene plasmid # 213154 ; http://n2t.net/addgene:213154 ; RRID:Addgene_213154)

  • For your References section:

    A Conserved Nonribosomal Peptide Synthetase in Xenorhabdus bovienii Produces Citrulline-Functionalized Lipopeptides. Li JH, Cho W, Hamchand R, Oh J, Crawford JM. J Nat Prod. 2021 Oct 22;84(10):2692-2699. doi: 10.1021/acs.jnatprod.1c00573. Epub 2021 Sep 28. 10.1021/acs.jnatprod.1c00573 PubMed 34581573
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