pCDF-Leup
(Plasmid #213154)

• Purpose: Expresses the leupeptin protease inhibitor pathway for bolstered production of heterologously expressed proteins.
• Depositing Lab: Jason Crawford
• Publication: Li et al J Nat Prod. 2021 Oct 22;84(10):2692-2699. doi: 10.1021/acs.jnatprod.1c00573. Epub 2021 Sep 28.

Backbone

• Vector backbone: pCDFDuet-1
• Backbone manufacturer: Novagen (EMD Millipore)
• Backbone size w/o insert (bp): 3781
• Total vector size (bp): 9421
• Modifications to backbone: Insert the Leupeptin Protease Inhibitor Pathway
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Streptomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Leupeptin Protease Inhibitor Pathway
• Alt name: LeupA, LeupB, LeupC, LeupD
• Species: Xenorhabdus bovienii SS-2004
• Insert Size (bp): 5733
• Promoter: T7

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: AvrII (not destroyed)
• 5′ sequencing primer: ACYCDuetUP1
• 3′ sequencing primer: DuetDOWN1

Resource Information

• Supplemental Documents:
  • pCDF-Leup.ape
• A portion of this plasmid was derived from a plasmid made by: Jhe-Hao Li

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry