pMXs-mRFP1
(Plasmid #21315)

• Purpose: MoMLV-based Retroviral vector, expresses mRFP1
• Depositing Lab: James Ellis
• Publication: Hotta et al Nat Methods. 2009 May . 6(5):370-6.

Backbone

• Vector backbone: pMXs MoMLV-based retroviral vector
• Backbone size w/o insert (bp): 4582
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: monomeric Red Fluorescence Protein 1
• Alt name: mRFP1
• Insert Size (bp): 754
• GenBank ID: AF506027

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: HincII/EcoRV (destroyed during cloning)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

mRFP1 (maximum Excitation wave length, 584 nm; maximum Emission wave length, 607 nm). pMXs MoMLV-based retroviral vector from Dr. Toshio Kitamura (Institute of Medical Science, University of Tokyo).