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Addgene

Skp
(Plasmid #213139)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 213139 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    The depositing lab uses BL21(DE3) for protein expression.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SKP (21-161)
  • Species
    E.coli
  • Insert Size (bp)
    420
  • Entrez Gene
    skp (a.k.a. b0178, ECK0177, JW0173, hlpA, ompH)
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

6xHis-tag -- TEV cleavage site -- SKP

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Skp was a gift from Sebastian Hiller (Addgene plasmid # 213139 ; http://n2t.net/addgene:213139 ; RRID:Addgene_213139)

  • For your References section:

    High-throughput screening of BAM inhibitors in native membrane environment. Rath P, Hermann A, Schaefer R, Agustoni E, Vonach JM, Siegrist M, Miscenic C, Tschumi A, Roth D, Bieniossek C, Hiller S. Nat Commun. 2023 Sep 13;14(1):5648. doi: 10.1038/s41467-023-41445-w. 10.1038/s41467-023-41445-w PubMed 37704632
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