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Addgene

SurA
(Plasmid #213138)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 213138 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    The depositing lab uses BL21(DE3) for protein expression.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SurA (21-428)
  • Species
    E.coli
  • Insert Size (bp)
    407
  • Entrez Gene
    surA (a.k.a. b0053, ECK0054)
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

6xHis-tag -- TEV cleavage site -- SurA

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SurA was a gift from Sebastian Hiller (Addgene plasmid # 213138 ; http://n2t.net/addgene:213138 ; RRID:Addgene_213138)

  • For your References section:

    High-throughput screening of BAM inhibitors in native membrane environment. Rath P, Hermann A, Schaefer R, Agustoni E, Vonach JM, Siegrist M, Miscenic C, Tschumi A, Roth D, Bieniossek C, Hiller S. Nat Commun. 2023 Sep 13;14(1):5648. doi: 10.1038/s41467-023-41445-w. 10.1038/s41467-023-41445-w PubMed 37704632
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