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Addgene

OmpT
(Plasmid #213137)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 213137 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-28 b (+)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    The depositing lab uses BL21(DE3) Omp8 for protein expression.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    OmpT
  • Species
    E.coli
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

OmpG-signal -- His-tag -- TEV-site -- OmpT

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    OmpT was a gift from Sebastian Hiller (Addgene plasmid # 213137 ; http://n2t.net/addgene:213137 ; RRID:Addgene_213137)

  • For your References section:

    High-throughput screening of BAM inhibitors in native membrane environment. Rath P, Hermann A, Schaefer R, Agustoni E, Vonach JM, Siegrist M, Miscenic C, Tschumi A, Roth D, Bieniossek C, Hiller S. Nat Commun. 2023 Sep 13;14(1):5648. doi: 10.1038/s41467-023-41445-w. 10.1038/s41467-023-41445-w PubMed 37704632
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