OmpX
(Plasmid
#213136)
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PurposeExpresses outer membrane protein X
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 213136 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET28b
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsThe depositing lab uses BL21(DE3) Omp8 for protein expression.
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameOmpX (24-171)
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SpeciesE.coli (K12)
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Insert Size (bp)441
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Entrez GeneompX (a.k.a. b0814, ECK0803, ompP, ybiG)
- Promoter T7
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
inclusion bodies
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
OmpX was a gift from Sebastian Hiller (Addgene plasmid # 213136 ; http://n2t.net/addgene:213136 ; RRID:Addgene_213136) -
For your References section:
The electrostatic core of the outer membrane protein X from E. coli. Rath P, Sharpe T, Hiller S. Biochim Biophys Acta Biomembr. 2020 Jan 1;1862(1):183031. doi: 10.1016/j.bbamem.2019.183031. Epub 2019 Jul 30. 10.1016/j.bbamem.2019.183031 PubMed 31374213
