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Addgene

OmpX
(Plasmid #213136)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 213136 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    The depositing lab uses BL21(DE3) Omp8 for protein expression.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    OmpX (24-171)
  • Species
    E.coli (K12)
  • Insert Size (bp)
    441
  • Entrez Gene
    ompX (a.k.a. b0814, ECK0803, ompP, ybiG)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

inclusion bodies

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    OmpX was a gift from Sebastian Hiller (Addgene plasmid # 213136 ; http://n2t.net/addgene:213136 ; RRID:Addgene_213136)

  • For your References section:

    The electrostatic core of the outer membrane protein X from E. coli. Rath P, Sharpe T, Hiller S. Biochim Biophys Acta Biomembr. 2020 Jan 1;1862(1):183031. doi: 10.1016/j.bbamem.2019.183031. Epub 2019 Jul 30. 10.1016/j.bbamem.2019.183031 PubMed 31374213
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