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pENTR-D-sp6-kcnk4b
(Plasmid #213123)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 213123 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pENTR-D-TOP
  • Backbone size w/o insert (bp) 2605
  • Total vector size (bp) 4060
  • Modifications to backbone
    Sp6 was added before attL1
  • Vector type
    Cloning

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    kcnk4b
  • Alt name
    LOC101882857
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    1455
  • Mutation
    Stop codon removed
  • GenBank ID
    XM_021481306.1
  • Entrez Gene
    LOC101882857
  • Promoter T7

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR-D-sp6-kcnk4b was a gift from GuangJun Zhang (Addgene plasmid # 213123 ; http://n2t.net/addgene:213123 ; RRID:Addgene_213123)

  • For your References section:

    Evolution of two-pore domain potassium channels and their gene expression in zebrafish embryos. Park SJ, Silic MR, Staab PL, Chen J, Zackschewski EL, Zhang G. Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.690. 10.1002/dvdy.690 PubMed 38270285
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