pENTR-D-sp6-kcnk4b
(Plasmid
#213123)
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PurposeRiboprobe generation
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 213123 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepENTR-D-TOP
- Backbone size w/o insert (bp) 2605
- Total vector size (bp) 4060
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Modifications to backboneSp6 was added before attL1
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Vector typeCloning
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namekcnk4b
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Alt nameLOC101882857
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)1455
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MutationStop codon removed
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GenBank IDXM_021481306.1
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Entrez GeneLOC101882857
- Promoter T7
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENTR-D-sp6-kcnk4b was a gift from GuangJun Zhang (Addgene plasmid # 213123 ; http://n2t.net/addgene:213123 ; RRID:Addgene_213123) -
For your References section:
Evolution of two-pore domain potassium channels and their gene expression in zebrafish embryos. Park SJ, Silic MR, Staab PL, Chen J, Zackschewski EL, Zhang G. Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.690. 10.1002/dvdy.690 PubMed 38270285
