POC1399
(Plasmid
#213120)
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Purpose(Empty Backbone) Testing the activity of BsaI-associated switch methylases
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 213120 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepL1R-1
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Backbone manufacturerNovoPro
- Backbone size (bp) 4968
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
POC1399 was a gift from Christopher A. O'Callaghan (Addgene plasmid # 213120 ; http://n2t.net/addgene:213120 ; RRID:Addgene_213120) -
For your References section:
DNA methylases for site-selective inhibition of type IIS restriction enzyme activity. Flores-Fernandez CN, Lin D, Robins K, O'Callaghan CA. Appl Microbiol Biotechnol. 2024 Jan 25;108(1):174. doi: 10.1007/s00253-024-13015-7. 10.1007/s00253-024-13015-7 PubMed 38270650