PL-SIN-PGK-EiP
(Plasmid #21312)

• Purpose: Control Lentiviral vector with ubiquitous PGK promoter, expresses EGFP and Puro resistance
• Depositing Lab: James Ellis
• Publication: Hotta et al Nat Methods. 2009 May . 6(5):370-6.

Backbone

• Vector backbone: PL-SIN lentiviral vector
• Backbone manufacturer: Buzina et al., PLoS Genetics, 2008
• Backbone size w/o insert (bp): 6141
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Enhanced Green Fluorescence Protein, Puromycin resistance gene
• Alt name: EGFP-IRES-Puro
• Insert Size (bp): 1947
• Promoter: PGK

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

EGFP and Puro resistance genes driven by PGK promoter. PL-SIN lentiviral vector (Buzina et al., PLoS Genetics, 2008) requires Tat cDNA for lentivirus production, as well as other packaging cDNAs (Gag/pol, Rev, and VSV-G).