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Addgene

pGL3-VEGFR2-570
(Plasmid #21309)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21309 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL3
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4818
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VEGFR2 promoter
  • Alt name
    VEGFR2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    838
  • Entrez Gene
    KDR (a.k.a. CD309, FLK1, VEGFR, VEGFR2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer RVprimer3
  • 3′ sequencing primer LucNrev
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

-570 to +268 region of the promoter.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3-VEGFR2-570 was a gift from Donald Ingber (Addgene plasmid # 21309 ; http://n2t.net/addgene:21309 ; RRID:Addgene_21309)

  • For your References section:

    A mechanosensitive transcriptional mechanism that controls angiogenesis. Mammoto A, Connor KM, Mammoto T, Yung CW, Huh D, Aderman CM, Mostoslavsky G, Smith LE, Ingber DE. Nature. 2009 Feb 26. 457(7233):1103-8. 10.1038/nature07765 PubMed 19242469
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