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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 21306 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGL3
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 4818
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Vector typeLuciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameVEGFR2 promoter
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Alt nameVEGFR2
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SpeciesH. sapiens (human)
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Insert Size (bp)493
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Entrez GeneKDR (a.k.a. CD309, FLK1, VEGFR, VEGFR2)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SacI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer RVprimer3
- 3′ sequencing primer LucNrev (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
-225 to +268 region of the promoter.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGL3-VEGFR2-225 was a gift from Donald Ingber (Addgene plasmid # 21306 ; http://n2t.net/addgene:21306 ; RRID:Addgene_21306) -
For your References section:
A mechanosensitive transcriptional mechanism that controls angiogenesis. Mammoto A, Connor KM, Mammoto T, Yung CW, Huh D, Aderman CM, Mostoslavsky G, Smith LE, Ingber DE. Nature. 2009 Feb 26. 457(7233):1103-8. 10.1038/nature07765 PubMed 19242469