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Addgene

pCR8-attL3-pegRNA-attL2
(Plasmid #213052)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 213052 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR8
  • Total vector size (bp) 4468
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sgRNA
  • gRNA/shRNA sequence
    GTTTAAGAGCTATGCTGGAAACAGCATAGCAAGTTTAAATAAGGCTAGTCCGTTATCAACTTGAAAAAGTGGCACCGAGTCGGTGC
  • Promoter 35S-CmYCLV-AtU6

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCR8-attL3-pegRNA-attL2 was a gift from Bing Yang (Addgene plasmid # 213052 ; http://n2t.net/addgene:213052 ; RRID:Addgene_213052)

  • For your References section:

    Modularly assembled multiplex prime editors for simultaneous editing of agronomically important genes in rice. Gupta A, Liu B, Raza S, Chen QJ, Yang B. Plant Commun. 2023 Oct 26:100741. doi: 10.1016/j.xplc.2023.100741. 10.1016/j.xplc.2023.100741 PubMed 37897041
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